Nanofluidic charged-coupled devices for controlled DNA transport and separation

Tuning transport properties of nanofluidic devices with local charge inversion.

Nanotubes can selectively conduct ions across membranes to make ionic devices with transport characteristics similar to biological ion channels and semiconductor electron devices. Depending on the surface charge profile of the nanopore, ohmic resistors, rectifiers, and diodes can be made. Here we show that a uniformly charged conical nanopore can have all these transport properties by changing ...

Micro/Nanofluidic Devices for Single Cells Analysis

1 Institute of Nano Engineering and Microsystems, National Tsing Hua University, Kuang Fu Road, No. 101, Section 2, Hsinchu 30013, Taiwan; E-Mail: [email protected] 2 Department of Engineering and System Science, National Tsing Hua University, Kuang Fu Road, No. 101, Section 2, Hsinchu 30013, Taiwan 3 Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Nankang, 11...

CHARGED POLYELECTROLYTE BRUSHES FOR VOLTAGE-CONTROLLED GATING OF NANOFLUIDIC CHANNEL: MOLECULAR DYNAMICS SIMULATION A Thesis

Restriction mapping in nanofluidic devices.

We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites wit...

Nanofluidic devices for rapid continuous-flow bioseparation.

Compared with conventional gel-based techniques, such as gel electrophoresis, which are routinely used for bioseparation in biology and biomedical laboratories, nanofluidic devices with regular engineered sieving structures offer the potential for faster separation, better resolution, higher throughput, and more convenient sample recovery. Here, we detail the fabrication process of a two-dimens...